Extended Data Fig. 21: Soma size and Spp1 status across spinal projecting neuron populations.
From: A transcriptomic taxonomy of mouse brain-wide spinal projecting neurons
a, Soma of SPNs were labelled with a retrograde AAV expressing GFP under a CAG promoter. Subsequently, IHC for Spp1 was performed, confocal images of each ROI were taken, and soma size / Spp1 status across each major ROI was measured. b, Proportion of Spp1+ SPNs across each ROI, as determined by IHC. c, Soma area of Spp1+ and Spp1- SPNs in ROIs that contained Spp1+ SPNs (i.e., CB, MB, MED, PONS regions quantified together; N = 260 Spp1+ and 756 Spp1- nuclei). d, Soma area of Spp1+ and Spp1- SPNs in ROIs that contained Spp1+ SPNs, separated by ROI (Spp1 + : N = 68 CB, 95 MB, 82 MED, 15 PONS; Spp1-: N = 4 CB, 152 MB, 312 MED, 288 PONS). Numbers above boxplots indicate p-values (Wilcoxon test, two-sided). The centre line of the box and whisker plots depicts the median value (50th percentile) while the box contains the 25th to 75th percentiles; the whiskers correspond to the 5th and 95th percentiles. e, Allen ISH Atlas data of Spp1, Pvalb, and Kcng4 in RN, DCN, Ve, and GRN (Allen Mouse Brain Atlas63, mouse.brain-map.org). RN, red nucleus; DCN, deep cerebellar nuclei; Ve, vestibular nucleus; GRN, gigantocellular reticular nucleus.
