Extended Data Fig. 9: Loss of OXT in TH+ neurons reduces lipolysis.
From: Control of lipolysis by a population of oxytocinergic sympathetic neurons
a. Scheme of the floxed Oxt allele. b. Co-immunostaining of OXT and TH in L1 sympathetic ganglia of Oxtflox and OxtΔTH mice. Scale bars, 50 um. c. Representative three-dimensional projection of cleared iWAT of Oxtflox and OxtΔTH mice co-immunolabeled by OXT (red) and TH (green). Scale bars, 200 um. d. Daily food intake of chow-fed Oxtflox and OxtΔTH mice; n = 7. e. Body weight of Oxtflox and OxtΔTH mice on chow and HFD; chow Oxtflox n = 7, chow OxtΔTH n = 7, HFD Oxtflox n = 9 and HFD OxtΔTH n = 10. f. Lean and fat mass of the Oxtflox (n = 6) and OxtΔTH (n = 7) mice on chow. g. Lean and fat mass of the Oxtflox (n = 5) and OxtΔTH mice (n = 7) on HFD. h. Tissue weight of liver, BAT, iWAT, and eWAT of Oxtflox (n = 5) and OxtΔTH (n = 7) mice on HFD for 16 weeks. Exact P value: eWAT, Oxtflox vs. OxtΔTH, P = 0.0448. i. Serum FFA levels from female Oxtflox and OxtΔTH mice housed at 4 °C at the indicated times; OxtΔTH at time point 0 n = 5, for the rest n = 6. Oxtflox vs. OxtΔTH at 0.5 h, P = 0.033; at 1 h, P = 0.0228. j. Serum FFA levels of female Oxtflox and OxtΔTH mice after ISO treatment; n = 6. ISO, Oxtflox vs. OxtΔTH, P = 0.0182. k. Western blotting of pPLIN1, PLIN1, pHSL and HSl of eWAT of Oxtflox and OxtΔTH mice housed at 4 °C for 1 week; n = 4. l. Norepinephrine levels in eWAT of Oxtflox and OxtΔTH mice housed at 4 °C for 1 week; n = 4. For d-l, data are from biologically independent samples. Data are presented as mean ± s.e.m. Statistical comparisons were made using 2-tailed Student’s t test (d, f-j and l) or 2-way ANOVA (e). *P < 0.05. For gel source data, see Supplementary Fig. 1.
