Extended Data Fig. 10: Human enhancer design by motif implantation.

a-b, Bar plots show the mean luciferase signal (log₂ fold-change over Renilla) of the synthetic sequences, which were generated by motif implantation, tested in MM001 (a, MEL melanoma cell line, n = 3 biological replicates) and MM047 (b, MES melanoma cell line, n = 2 biological replicates). Values of 2 previously validated MES regions are displayed for MM047. The error bars in a, show the standard error of the mean. c, Nucleotide contribution scores of the selected synthetic sequences in their initial form (first row), after adding SOX10, MITF and TFAP2 motifs once (second row), after adding SOX10, MITF and TFAP2 motifs twice (third row), weaker-motif version of the third row after replacing implanted motifs with weaker sites (fourth row), cut version of the second row where only the part with the binding sites were taken (fifth row, left) and minimal version of the second row where MITF and TFAP2 are placed as close as possible to SOX10 (fifth row, right). The names of the motifs and their implantation order are indicated at the top. The position of the motifs is shown with dashed lines.