Extended Data Fig. 1: Spatial transcriptomics resolves the engram assembly in different neuronal cell types.
From: Spatial transcriptomics reveal neuron–astrocyte synergy in long-term memory
a) Engram cells (tdTomato +) revealed by MERFISH. b-i) Quantification of tdTomato+ neurons in all regions (b, n [FR] = 5 mice, n [NF] = 4 mice), retrosplenial area (RSP, c, n [FR] = 7 sections, n [NF] = 7 sections), paraventricular nucleus of the thalamus (PVT, d, n [FR] = 7 sections, n [NF] = 6 sections), ventral posterior complex of thalamus (VP, e, n [FR] = 10 sections, n [NF] = 11 sections), hippocampus (HIP, f, n [FR] = 8 sections, n [NF] = 9 sections), basolateral amygdala (BLA, g, n [FR] = 8 sections, n [NF] = 7 sections), central amygdala (CeA, h, n [FR] = 9 sections, n [NF] = 10 sections), and zona incerta (ZI, i, n [FR] = 10 sections, n [NF] = 10 sections), mean +/- S.E.M, unpaired two-tailed student t-test. j) Unbiased clustering of all cells resolved in situ. k) Marker genes expression of major cell types. l) Major cell types with annotations resolved a UMAP. m) All cells grouped by HC, FR, and NF conditions. All MERFISH data.
