Extended Data Fig. 2: Spatial transcriptomics resolves memory associated genes.
From: Spatial transcriptomics reveal neuron–astrocyte synergy in long-term memory
a) Marker genes expression of neuronal cell types. Neurons grouped by HC, FR, and NF conditions. b) Differentially gene expression analysis of peri-engram neurons (neurons within a radius of 30 um to engram neurons) other neurons. c) Genes enriched in peri-engram neurons over other neurons, unadjusted P value by Mann Whitney Wilcoxon test. d) Engram neurons and peri-engram neurons resolved in situ. e) Unbiased clustering of all cells from BLA. f) Marker genes expression of major cell types in the BLA. g) Fear memory induced gene expression in excitatory engram neurons of BLA, FR vs. HC. h) Fear memory induced gene expression in inhibitory engram neurons of BLA, FR vs. HC. i) BLA neurons grouped by FR and NF conditions. j) Penk to Tac2 ratio of all neurons in BLA. k) Penk to Tac2 ratio of TRAPed neurons in BLA, one-way ANOVA and two-sided Mann Whitney Wilcoxon test. l) Penk to Tac2 ratio of TRAPed inhibitory neurons in BLA, one-way ANOVA and two-sided Mann Whitney Wilcoxon test. All MERFISH data.
