Extended Data Fig. 11: TDP-43 dose-dependent toxicity and upregulation of NPTX2.
From: A model of human neural networks reveals NPTX2 pathology in ALS and FTLD
a, Immunofluorescence of iNets expressing TDP-43-HA without or with its 3’UTR or HA-FUS driven by the inducible TRE or constitutive EF1α promoter for 1 week or 2 weeks revealing specificity of NPTX2 accumulation to TDP-43 dysregulation. Scale bars, 20 µm. b, Quantification of averaged mean intensities of nuclear TDP-43-HA signal of TDP-43-HA+ cells in iNets from the 1 week condition in (a). One-way ANOVA followed by Tukey’s multiple comparisons test (mean of each dataset compared with the mean of every other dataset). Data shown are mean with SD. TRE-TDP-43-HA vs EF1α-TDP-43-HA; and vs EF1α-TDP-43-HA-3’UTR p < 0.0001; EF1α-TDP-43-HA vs EF1α-TDP-43-HA-3’UTR p < 0.0001. Each data point represents the average intensity value measured from all TDP-43-HA+ nuclei detected in a well (36 fields of view; 2852-3749 cells measured per condition).
