Extended Data Fig. 3: Schematics of network and single-cell properties and individual plots from data shown in Fig. 1l–n.
From: A model of human neural networks reveals NPTX2 pathology in ALS and FTLD
a, Timeline of the experiment: starting from iCoMoNSCs (0 months), iNets were differentiated, sequenced and plated on MEAs at young (1.5 months), middle (3 months) and old (7 months) stages. Created with BioRender.com. b, HD-MEA chip mounted on a printed circuit board featuring a cell culture chamber (ring) and the microelectrode array in the center (green). Image courtesy of MaxWell Biosystems AG, print permitted. Scale bar, 4 mm. c, Representative spike time histogram recorded from 1,020 electrodes used to illustrate how network metrics were extracted. d, Representative spontaneous APs recorded from one electrode, used to illustrate how single-cell metrics were extracted. e, Representative action potential (AP) used to illustrate how features were extracted from the AP shape. Panels c-e serve illustrative purposes only. f-t, Box plots representing all results illustrated in Fig. 1l, m. Each data point represents the respective metric value from one HD-MEA culture (n = 7 (young), 10 (middle), 10 (old)). Box plots indicate distribution median value and the 25th and 75th percentiles. f, BD Young vs Middle p < 0.007; h, IBIcv Young vs Middle p = 0.015; j, AE Young vs Old p = 0.0005; l, ISIcv Young vs Old p = 0.004; m, MFR Young vs Old p < 0.025; p, PtV Young vs Middle p = 0.02 and Young vs Old p = 0.003; t, BL Young vs Middle p = 0.008 and Young vs Old p = 0.001. u, Principal component analysis (PCA) demonstrating separation of the three maturation stages.
