Extended Data Fig. 3: Cryo-EM processing and reconstruction of the CINA-bound and PAM-free CaSR–Gi complexes in lipid nanodiscs.
From: Allosteric modulation and G-protein selectivity of the Ca2+-sensing receptor

a, Cryo-EM data processing workflow for the CINA-bound CaSR–Gi complex in nanodiscs. b, Representative cryo-EM micrograph (out of 20528 similar micrographs) and 2D averages for the CINA-bound CaSR–Gi complex in nanodiscs. c, Local refinement maps of VFT–CRD, CRD–7TM and Gαiβγ for the CINA-bound CaSR–Gi complex in nanodiscs with corresponding Fourier shell correlation (FSC) curves indicating nominal resolutions using the FSC = 0.143 criterion. d, Angular distribution heat map of particle projections for the global reconstruction of the CINA-bound CaSR–Gi complex in nanodiscs. e, Cryo-EM data processing workflow for the PAM-free CaSR–Gi complex in nanodiscs. f, Representative cryo-EM micrograph (out of 31047 similar micrographs) and 2D averages for the PAM-free CaSR–Gi complex in nanodiscs. g, Local refinement maps of VFT–CRD, CRD–7TM and Gαiβγ for the PAM-free CaSR–Gi complex in nanodiscs with corresponding Fourier shell correlation (FSC) curves indicating nominal resolutions using the FSC = 0.143 criterion. h, Angular distribution heat map of particle projections for the global reconstruction of the PAM-free CaSR–Gi complex in nanodiscs. i, Local refinement maps of VFT–CRD, CRD–7TM for the PAM-free active-state CaSR in nanodiscs with corresponding FSC curves indicating nominal resolutions using the FSC = 0.143 criterion. j, Angular distribution heat map of particle projections for global reconstruction of the PAM-free active-state CaSR in nanodiscs.