Extended Data Fig. 9: Comparison of C. albicans WT and ece1Δ/Δ cells in vitro.
From: The hyphal-specific toxin candidalysin promotes fungal gut commensalism
a, Heat map of transcriptome analysis representing the fold change (FC) in expression of fungal genes when strains were grown under hyphal-inducing conditions (3 h, 37 °C, 5% CO2). b-h, Competitive fitness of SC5314 WT and ece1Δ/Δ cells in RPMI (37 °C, 5% CO2) (b), acidic pH (pH 4.7) (c), high salt (1 M NaCl) (d), hypoxia (1% O2) (e), oxidative stress (2 mM H2O2) (f), in the presence of 2% acetate (g), and during incubation with TR146 epithelial cells (h). Competitive index was calculated using the formula: log2 [(MUTcompetition/WTcompetition)/ (MUTsingle/WTsingle)]. Fitness of the ece1Δ/Δ relative to the WT is represented as log2 competitive index. Data are representative of three biological replicates (n = 3). Graphs show the mean ± sem in b - h. Statistical analysis was performed using 1-way ANOVA with Bonferroni post hoc test to detect significance.
