Fig. 4: Pathogen-induced RBA1 condensates mediate cell death in Arabidopsis.
From: Substrate-induced condensation activates plant TIR ___domain proteins
a,b, Confocal images of RBA1p:RBA1/Col-0 transgenic lines (no. 3 and no. 8) (a) and RBA1p:RBA1BB-loop/Col-0 transgenic lines (no. 6 and no. 12) (b) Arabidopsis plants 24 h after inoculation with Pf0-1 with empty vector (EV) or expressing HopBA1. The fluorescence intensity of the images was calibrated by the autofluorescence in the guard cells. Ten leaves from five plants were detected for each treatment, showing similar results. Scale bars, 40 μm. c, Western blot analysis of total leaf protein extracts of transgenic RBA1p:RBA1/Col-0 and RBA1p:RBA1BB-loop/Col-0 Arabidopsis plants 24 h after inoculation. RBA1–GFP and RBA1(BB-loop)–GFP were probed with GFP antibody. Ponceau staining was used to indicate equal loading of samples. BA, inoculation with Pf0-1 expressing HopBA1; EV, inoculation with Pf0-1 expressing empty vector; −, no inoculation. Experiments were repeated at least three times with similar results. d, Visual signs of cell death in leaves of transgenic RBA1p:RBA1/Col-0 and RBA1p:RBA1BB-loop/Col-0 Arabidopsis plants 36 h after inoculation with Pf0-1 expressing empty vector (EV) or HopBA1. The numbers on the bottom indicate the proportion of leaves exhibiting cell death out of all inoculated leaves.
