Extended Data Fig. 2: HAT enzyme expression and regulation of astrocyte epigenetic memory.
From: Disease-associated astrocyte epigenetic memory promotes CNS pathology
(a) EP300, Tip60 (KAT5), and PCAF (KAT2B) signaling network in isolated brain astrocytes that received ICV administration of IL-1β/TNF twice (2X) compared to once (1X). (b) qPCR analysis of Ep300, Kat5, and Kat2b expression in primary astrocytes stimulated after 30 min IL-1β/TNF stimulation on day 7 (n = 4 per group). Unpaired two-sided t-test. (c) Primary astrocytes were stimulated with IL-1β/TNF once (1X), twice (2X), or three times (3X). qPCR analysis of astrocytes after 30 min activation with IL-1β/TNF on day 14 (n = 4 per group). Unpaired two-sided t-test. (d) Experimental design for (e). (e) qPCR of primary astrocytes in the presence with/without C646 (p300/CBP inhibitor), MB-3 (Gcn5/PCAF inhibitor), and MG149 (Tip60 inhibitor) after 30 min stimulation with IL-1β/TNF on day 7 (n = 5 per group). Unpaired two-sided t-test. (f) Experimental design for Fig. 1j. (g) Gene expression 30 min after IL-1β/TNF stimulation on day 7 of EGFP positive/negative astrocytes treated with C646 or vehicle (n = 8 per group). Unpaired two-sided t-test. Data shown as mean ± s.e.m.
