Extended Data Fig. 3: Cryo-EM data processing of Pol II Rat1-PTTC2. | Nature

Extended Data Fig. 3: Cryo-EM data processing of Pol II Rat1-PTTC2.

From: Structural basis of exoribonuclease-mediated mRNA transcription termination

Extended Data Fig. 3

a, The flowchart of data processing for Pol II Rat1-PTTC2. b-c, Gold-standard FSC curves of map 2a, map 2b. d, The 3D FSC plot. The dotted line represents the 0.143 cutoff of the global FSC curve. e, Nominal 2.8 Ã…-resolution cryo-EM reconstruction of map 2a filtered by local resolution. f, Local refined map (map 2b) of Rat1, Rai1, Rpb4-Rpb7, and structure elements around the RNA exit channel of Pol II.

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