Extended Data Fig. 6: Data processing of OSCA3.1 in detergent, nanodiscs, and liposomes.
From: Mechanical activation opens a lipid-lined pore in OSCA ion channels
a-g, Image-processing workflow of OSCA3.1-GDN (a), OSCA3.1-azolectin-1:40 (b), OSCA3.1-azolectin-1:20 (c), OSCA3.1-azolectin-1:10 (d), OSCA3.1-PG10-1:20 (e), OSCA3.1-LPC9-1:20 (f), and OSCA3.1-liposome (g) with Relion and CryoSPARC. Gold-standard FSC curves (FSC = 0.143) are displayed beside the refined maps. The atomic models of OSCA3.1 in closed (red) and open (green) states are fitted into the obtained OSCA3.1-inside-in structure, thereby showing that the structure is in a closed state (g). h, The particle distribution of OSCA3.1 in different conformations. AZO, azolectin. i, A Zoom-in of a dimer of dimer class obtained during 3D classification in OSCA3.1-GDN dataset (a). The top view of the cryo-EM map (left) and the side view of the model (right) shows the tetramer consists of two dimers, colored in gold and cyan, respectively.
