Extended Data Fig. 7: The lipids in cryo-EM maps of OSCA channels.
From: Mechanical activation opens a lipid-lined pore in OSCA ion channels
a, Lipid and acyl chain densities (magenta) are visible in cryo-EM maps of OSCA channels in both detergent and nanodiscs. b, The pore exit lipids (magenta) in OSCA1.2 closed subunit (OSAC1.2-DOPC-1:20-contracted1 and OSCA1.2-liposome-inside-out-closed) and ‘activated’ subunit (OSAC1.2-DOPC-1:20-contracted2 and OSCA1.2-liposome-inside-in-open). c, The lipid bound in the lower cleft of OSCA3.1 in detergent and nanodiscs. d, The charged residues in the lower cleft of OSCA channels. The cyan circles indicate the unique positive charged residues in OSCA3.1 as compared to OSCA1.1 and OSCA1.2. The red circles indicate the unique negative charged residues in OSCA1.1 and OSCA1.2 as compared to OSCA3.1. e, Sequence alignment of residues on cleft facing helices in OSCA3.1, OSCA1.1 and OSCA1.2. The unique positive charged residues (cyan) in OSCA3.1 and unique negative charged residues (red) in OSCA1.1 and OSCA1.2 are highlighted. There residues are circled in (d). The residues sandwiching the bound lipid in OSCA3.1 are shown in blue boxes.
