Extended Data Fig. 12: Effect of β-arrestin-1 on smFRET distribution of phosphorylated µOR-Cy3/Cy5.
From: Ligand efficacy modulates conformational dynamics of the µ-opioid receptor
a, Phosphorylation of µOR∆7-182 C/276 C by GRK5 for DEER spectroscopy. Anion exchange chromatography (MonoQ) was used to find the best condition for GRK5 phosphorylation of the µOR. b, smFRET distributions of GRK5-phosphorylated µOR∆7-182 C/273C-Cy3/Cy5 (µORp-Cy3/Cy5) in the presence of 20 µM of C8-PIP2. The grey shaded areas are the DAMGO alone condition for comparison.
