Fig. 2: The antiviral DNA glycosylase Brig1 targets α-glucosyl-hmC nucleobases to restrict T4 viral replication.
From: DNA glycosylases provide antiviral defence in prokaryotes
a, Tenfold serial dilutions of different T4 phage stocks on lawns of E. coli EC100, each lawn carrying cosmid pWEB-TNC or pBrig1, and plasmids pEmpty, p(a-gt) or p(b-gt). Plaque images of one representative experiment from three independent experiments are shown. b, Normalized next-generation sequencing reads of T4 escaper1 DNA, mapped to the viral genome. DNA for sequencing was obtained 8 min after infection of E. coli EC100 carrying pWEB-TNC or pBrig1 at an MOI of 5. c, Tenfold serial dilutions of different T4 phage stocks on lawns of E. coli EC100, each lawn carrying cosmid pWEB-TNC or pBrig1, and plasmid pEmpty, p(a-gt) or p(gp42). Plaque images of one representative experiment from three independent experiments are shown.
