Extended Data Fig. 2: Optimization of P450 oxidation efficiency through the expression of a scaffolding MSBP.

a, Phylogenetic tree of Saponaria vaccaria MSBP homologues and related MSBPs (see details in the Supplementary Methods). b, Expression of various MSBP homologues, including 2 newly identified SvMSBPs, led to 2- to 4-fold increase of QA production. Increased of QA production can be attributed to the improved oxidation efficiency. Data are mean ± s.d.; n = 3 biologically independent samples. *P value < 0.05; ** P < 0.01. Student’s two-tailed t-test. More statistical analysis is available in the source data file. Subcellular localization studies show that SvMSBP1 co-localizes with both c, C28 and d, C23 oxidases on the ER membrane of yeast. Images were acquired using a Zeiss LSM 710 confocal microscope (scale bar represents 10 μm; at least three independent experiments were conducted).