Extended Data Fig. 7: Analysis of the 3D reconstructions of the mutant Vγ9Vδ2 and mutant Vγ5Vδ1 TCR–CD3 complexes.

a-b, Gold standard FSC curves (a) and local resolution estimation (b) of 3D reconstructions of TMα mutant Vγ9Vδ2 TCR–CD3 complex (left), AAA mutant Vγ9Vδ2 TCR–CD3 complex (middle), and EH mutant Vγ5Vδ1 TCR–CD3 complex (right). Maps were sharpened by DeepEMhancer. c, Angular distribution of the TMα mutant Vγ9Vδ2 TCR–CD3 complex (left), AAA mutant Vγ9Vδ2 TCR–CD3 complex (middle), and EH mutant Vγ5Vδ1 TCR–CD3 complex (right) complex particles in the final round of 3D refinement using CryoSPARC. d, Model versus map comparison for the final 3D reconstruction of the TMα mutant Vγ9Vδ2 TCR–CD3 complex (left), AAA mutant Vγ9Vδ2 TCR–CD3 complex (middle), and EH mutant Vγ5Vδ1 TCR–CD3 complex (right). FSC curves of the refined models are compared with the overall refinement maps (black), as well as to compare each model refined in one of the two independent maps used for the FSC with the same map (red), and with the second independent map (green). The marginal discrepancy between the red and green curves suggests that the model refinement process was not significantly affected by overfitting. e, The 3DFSC sphericity plot for the final 3D reconstruction of the TMα mutant Vγ9Vδ2 TCR–CD3 complex (left), AAA mutant Vγ9Vδ2 TCR–CD3 complex (middle), and EH mutant Vγ5Vδ1 TCR–CD3 complex (right). This plot was generated using 3DFSC.