Fig. 4: [Ca²⁺]_i oscillations in pollen grains prior to germination are impaired in osca2.1/2.2.

a,b, Representative traces of GCaMP6 fluorescence recorded in a wild-type (a) or an osca2.1/2.2 (b) pollen grain. Pollen expressing GCaMP6 were placed on standard germination medium and fluorescence images were taken every 30 s for 300 min. GCaMP6 images (scaled with a pseudocolor bar) at indicated time points are shown. The resting phases (RePh1, RePh2 and RePh3) were separated by [Ca²⁺]_i oscillations with small amplitudes (CaOsc^S) or large amplitudes (CaOsc^L). P_L, peak CaOsc^L amplitude; P_S, peak CaOsc^S amplitude; T_L, trough CaOsc^L amplitude; T_S, trough CaOsc^S amplitude. Arrowheads indicate the germination aperture. Vertical scale bar (panel a, top left): the whole scale is 0.5 r.u with 0.1 r.u. divisions. Similar results were seen more than 20 times.

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