Extended Data Fig. 5: Lack of epithelial contribution to gut shape phenotypes.
a,b, bnl downregulation from gut enterocytes (mex1>bnlRNAi) does not reduce tracheal branches (QF6>mtdTomato) (top (a), quantified in left (b)), or number of DSRF-positive nuclei (bottom (a), quantified in right (b)). c-d, btl downregulation from gut epithelium for 3 days prior to pupal eclosion (mex1,esgTS>btlRNAi) does not reduce tracheal branches (QF6>mtdTomato) (top (c), quantified in left (d)), or number of DSRF-positive nuclei (bottom (c), quantified in right (d)). e, PCA plot of mex1>bnlRNAi gut shape variability showing no significant difference in shape to controls. f, Average mex1>bnlRNAi gut centrelines show no differences in shape relative to controls. g, PCA plot of mex1,esgTS>btlRNAi gut shape variability showing no significant difference in shape to controls. h, Average mex1,esgTS>btlRNAi gut centrelines show no significant differences in shape relative to controls. Boxplots: line = median, box = first quartile and third quartile, whiskers = minimum and maximum. PCA plots: ellipses represent 95% confidence space for each group. Diagrams represent the extremes of variation along each PC (see methods). n = number of biologically independent samples. Statistical significance was assessed using one-way ANOVA followed by Tukey post-hoc tests (b,d): non-significant (n.s.) = P > 0.05. See Supplementary Information for exact P-values, statistical tests and sample sizes. Males are in blue, females in orange and controls in lighter matching colours. Ctrl = control group (see genotypes in Supplementary Information). Scale bars: 200 μm.
