Fig. 5: Physiological importance of gut trachea.
a,b, Expression of bnlRNAi from gut muscle (Hand>bnlRNAi) does not change amounts of SimaODD::GFP (top of a, quantified in top of b) nor Ldh::GFP (bottom of a, quantified in bottom of b). c, Expression of bnlRNAi from gut muscles (Hand>bnlRNAi) increases number of mitoses in 20-day-old flies, seen by number of pH 3-positive cells. d, Hand>bnlRNAi expression in DSS-treated guts reduces mitotic indices in midgut of 7-day-old females relative to DSS-treated controls, seen by number of pH 3-positive cells. e,f, Expression of Hand>bnlRNAi (e) or Hand>SxlRNAi (f) reduces fecundity in females relative to controls, as measured by the number of laid eggs per fly on days 2, 5 and 8 after mating. GFP intensity and GFP/RFP ratio graphs show mean and standard deviation. Boxplots: line, median; box, first quartile and third quartile; whiskers, minimum and maximum. n denotes number of biologically independent samples. Statistical significance was assessed using one-way ANOVA followed by Tukey post hoc tests (c–f). *P < 0.05; **P < 0.01; ***P < 0.001. See Supplementary Information for exact P values, statistical tests and sample sizes. Males, blue; females, orange; controls, lighter matching colours (see genotypes in Supplementary Information). Scale bars, 200 μm.
