Extended Data Fig. 4: Expression of bnl and gut muscle drivers.
a, bnl expression (bnl-Gal4>StingerGFP) in the gut epithelium and muscles at different stages of development: in larval enterocytes (top left), in adult gut muscle at 5 h after pupal eclosion (APE) (middle left) and in adult enterocytes at 7 days APE (bottom left). Schematic shows bnl locus and the position of T2A-Gal4 insertion (top right). GFP intensity quantified at 5 h APE (middle right) and 7 days APE (bottom right). b, Histogram of Pearson correlation coefficients between gut curvature and bnl intensity along midgut. Grey bars represent correlations with a “control” bnl intensity generated by fitting an autoregressive process to the true data (see Methods). c, bnl expression (bnl-Gal4>StingerGFP) at 5 h APE in gut R3 region showing enterocyte expression (top) and R2 region showing muscle expression (bottom). d, bnl expression (bnl-Gal4 > StingerGFP) overlaps F-actin-rich muscle layer, shown by Phalloidin staining (Phal), but not intestinal stem cells or enteroblasts, shown by horseradish peroxidase staining (HRP), nor enteroendocrine cells, shown by Prospero staining (Pros). e, Hand expression (Hand-Gal4>myr::GFP) in: adult brain, ventral nerve cord, heart, indirect flight muscle, fat body, crop, hindgut, ovaries and testes. f, vm expression (vm-Gal4>myr::GFP) in: adult brain, ventral nerve cord, heart, Malpighian tubules, fat body, crop, hindgut, ovaries and testes. Line graphs: mean and standard deviation. n = number of biologically independent samples. See Supplementary Information for exact P-values, statistical tests and sample sizes. Males are in blue and females in orange. Scale bars: (a,c) 200 μm, (d) 50 μm, (e,f) 100 μm.
