Extended Data Fig. 1: RAG scanning for primary Vκ-to-Jκ1 rearrangement is terminated within Sis. Related to Fig. 2.
From: Molecular basis for differential Igk versus Igh V(D)J joining mechanisms
a, Diagram of single Jκ1 allele v-Abl line. b, Relative utilization percentage of individual Vκs in single Jκ1 allele line with Jκ1 bait. c, Percentage of pooled RAG off-target junctions in Igκ locus from single Jκ1 allele line. Right panel: zoom-in to the region between Cer and Jκ, highlighted in yellow. d, Percentage of inversional (red) and deletional (blue) cryptic RSS junctions within indicated Vκ locus (chr6:67,495,000-70,657,000) and Cer-Jκ regions (chr6:70,657,000- 70,674,500) from single Jκ1 allele line. Vκ utilization and cryptic RSS data are presented as mean ± s.e.m. from 3 biological repeats. Overall figure presentation is as described in Fig. 2. Note that the total on-target and off-target Jκ1 junctions recovered are, respectively, 5-fold and 8-fold greater than those recovered with a Jκ5 bait, consistent with the greater strength of the Jκ1-RSS47.
