Extended Data Fig. 6: Effects of restoring MYCT1 expression on human HSPCs in culture.
From: MYCT1 controls environmental sensing in human haematopoietic stem cells
a Relative MYCT1 expression by RT-qPCR evaluating in sorted control or MYCT1 OE CB HSPCs (CD34+CD38−CD90+GFP+) 72 h after transduction. n = 3 independent experiments, mean±s.e.m. b Representative FACS plots and gating strategy (b), and percentage (c) of undifferentiated CD34+CD38− cells, HSPCs (CD34+CD38−CD90+CD45RA−), and immunophenotypic LT-HSC (CD34+CD38−CD90+CD45RA−EPCR+ITGA3+) expanded from control or MYCT1 OE CB HSPCs after 10, 15 and 20 days in culture. Corresponds to quantifications in Fig. 3b. Percentage of cells in each population within the total cells is indicated in the FACS plots. n = 6 replicates from 3 independent experiments, mean±s.e.m, ratio-paired two-tailed t test. d Number and type of colonies formed in enriched methylcellulose (see Methods) after plating equal number of control or MYCT1 OE CB HSPCs (CD34+CD38−CD90+GFP+) sorted 72-96 h after transduction. CFU/BFU-E: Colony-forming unit-erythroid or burst-forming unit-erythroid erythroid, CFU-GM: granulocyte and/or macrophage, CFU-Mixed: granulocyte, erythroid, macrophage. n = 4 independent experiments, mean±s.e.m, paired two-tailed t test. e Percentage of cells that undergo 1 to 4 divisions, or no divisions observed for every 24 h elapsed since plating. n = 3 independent experiments, mean±s.e.m.
