Extended Data Fig. 7: Multilineage differentiation after transplantation of HSPCs with restored MYCT1 expression.
From: MYCT1 controls environmental sensing in human haematopoietic stem cells
a Table indicating the number of mice transplanted with control or MYCT1 OE HSPC for each condition per replicate, for the transplantations performed 96 h after transduction. b-e Percentage of human undifferentiated HSPC (CD34+CD38−) (b), hematopoietic progenitor cells (HPC, CD38+) (c), erythroid (CD71+GlyA+) (d), and megakaryocytes (CD41a+) (e) in BM from NBSGW mice 12 weeks after transplantation with 2500 HSPC. n = 17 mice per group. Median and all individual values are shown, two-tailed Mann–Whitney test. f Gating strategy and representative FACS plots showing human myeloid (CD14+ or CD66b+), B-lymphoid (CD19+), T-lymphoid/other (CD3, CD4 or CD8+), undifferentiated HSPC (CD34+CD38−), HPC (CD38+), megakaryocytes (CD41a+), and erythroid (GlyA+CD71+) populations of mice transplanted with 500 or 2500 HSPCs 96 h after transduction. Percentage of cells in each population within the total BM hematopoietic compartment (human and mouse CD45) is indicated in the FACS plots.
