Extended Data Fig. 10: Expression of MYCT1 and MYCT1 associated “stemness” programs in HSCs with low, medium or high endocytosis.
From: MYCT1 controls environmental sensing in human haematopoietic stem cells
a Relative expression of MYCT1 by RT-qPCR in highly purified 4-day cultured HSPCs (CD34+CD38−CD90+EPCR+) with low, medium or high internalization of fluorescent dextran (10 KDa). n = 3 independent experiments, mean±s.e.m. two-tailed t-test. b-g Single cell RNAseq dot plots depicting gene expression for the programmes that were found to be governed by MYCT1. HLF+ HSC cultured for 4 days and sorted for low, medium, or high internalization of fluorescent dextran (green box) are compared to control, MYCT1 KD and MYCT1 OE HLF+ (grey box) (From Fig. 2 and Extended Data Fig. 4). n = 1 experiment. The number of cells sequenced per fraction are shown in Supplementary Table 7.
