Extended Data Fig. 3: Documentation of the effects of MYCT1 knockdown on human foetal liver HSPC expansion and engraftment.
From: MYCT1 controls environmental sensing in human haematopoietic stem cells
a,b Quantifying expansion of foetal liver (FL) HSPC after MYCT1 KD. Fold expansion (a), representative FACS plots and percentage (b) of all live cells and immunophenotypic self-renewing HSCs (CD34+CD38−CD90+GPI80+) from control or MYCT1 KD FL. n = 6 (ctrl), n = 5 (KD1) and n = 4 (KD2) replicates from 2 independent experiments, mean±s.e.m. c,d Determining the transplantation ability of MYCT1 KD FL HSPCs. c FACS plots showing control or MYCT1 KD CB HSPCs sorted for transplantation 72 h after transduction. The gate indicates the population (CD34+CD38−CD90+GPI80+) that was sorted and transplanted. 10,000 sorted control or KD HSPCs were transplanted per mouse. d Representative FACS plots and quantification of human hematopoietic engraftment (hCD45+) and multilineage differentiation in NSG mice 24 weeks after transplantation. In FACS plots, percentage within total hCD45+ cells indicated. Quantification shows bone marrow at 6, 12 and 24 weeks after transplantation, and spleen and blood 24 weeks after transplantation. n = 5 (control) and n = 4 (MYCT1 KD) mice per group, mean±s.e.m., two-tailed Mann–Whitney test.
