Extended Data Fig. 4: Analysis of MYCT1 dependent programs in cultured human CB HSCs.
From: MYCT1 controls environmental sensing in human haematopoietic stem cells
a Experimental outline for scRNAseq analysis evaluating the effects of MYCT1 KD and MYCT1 OE in CB HSPCs 72 h after transduction (4 days in culture). CB HSPCs (CD34+CD38−CD90+) were isolated freshly or 72 h after transduction. b Number of total HSPCs and HLF+ cells sequenced for scRNAseq for each sample. c Percentage of HLF+ HSC among uncultured and cultured control, MYCT KD and OE HSPC. d,e TSNE plots showing all the sequenced HSPCs (d), or the selected HLF+ cells 72 h after transduction (e). f,g Dot plot depicting gene expression of selected human HSC signature genes18,33,34,35,36 that are significantly differentially expressed in HLF+ HSCs from MYCT1 KD and/or MYCT1 OE compared to control. Comparison of uncultured and cultured HLF+ HSCs is also shown. h-k scRNAseq dot plots depicting expression for selected genes in the programs found to be differentially regulated by MYCT1 KD or OE in HLF+ HSC (see Fig. 2b). n = 1 experiment with the indicated number of single cells sequenced. Schematic in a was created with BioRender (https://www.biorender.com).
