Extended Data Fig. 10: A rejuvenative gene therapy reestablishes the tripartite barrier to restore walking.
From: Single-cell and spatial atlases of spinal cord injury in the Tabulae Paralytica

a, Left, experimental design of a gene therapy intervention to promote the formation of the tripartite barrier, reproduced from Fig. 8a. Right, a second chronophotography series showing walking in old mice without (top) and with (bottom) a gene therapy intervention to promote the formation of the tripartite barrier. b, Composite tiled scans of GFAP and CD45 in horizontal sections from representative old and treated mice. c, Horizontal sections from representative old and treated mice identifying a restoration of Sox9ONId3ON cells in the astrocyte border region in treated mice. d, Composite tiled scans and confocal insets of albumin and GFAP in horizontal sections from representative old and treated mice after SCI. e, Line graph demonstrates albumin intensity at specific distances rostral and caudal to lesion centers. Bottom right, bar graph indicates the area under the curve (independent samples two-tailed t-test, n = 5 per group, t = 4.07, p = 0.0099). f, Locomotor performance in the gene therapy experiment, as quantified in Supplementary Fig. 3 (n > 10 gait cycles per mouse, (n = 5 mice per group; Tukey’s honestly significant difference test). *, p < 0.05; **, p < 0.01; ***, p < 0.001. g, Left, schematic overview of the classification pipeline using high-resolution kinematics data from young and old mice. Right, experimental conditions assigned to individual steps in old mice that received gene therapy by a machine-learning model trained on kinematics data from untreated animals.