Extended Data Fig. 11: Direct effects of anti-PD-1 treatment on tumor conditioned macrophage function and metabolism.
From: Obesity induces PD-1 on macrophages to suppress anti-tumour immunity
a, OCR from MitoStress Test of BMDM stimulated with TCM for 24 h followed by treatment with recombinant mouse PD-L1 and/or anti-PD-1 for an additional 24 h. b,c, Percent positive phrodo, and CD80 of TCM stimulated BMDM following rmPD-L1 and/or anti-PD-1 treatment. d-f, Percent positive phrodo, CD80 and MHCII of TAMs from MACS isolated CD11b+ cells from MC38 tumors ex-vivo treated α−PD-1 for 24 h. g, Fold change of MHCII, MHCI, Glut1, and iNOS expression of IgG/a-PD-1 ex-vivo treated TAMs from LFD versus HFD treated MC38 tumor bearing mice. h, Volcano plot of differentially expressed genes from MACS isolated CD11b+ cells from MC38 tumors ex-vivo treated α-PD-1 for 24 h. Data points indicated biological replicates. Data in a-c representative of ≥ three independent experiments with ≥ 3 mice per group run in triplicate. Data are mean ± s.e.m. P values were calculated using a One-way ANOVA (a-c), a paired two-tailed t-test (d-f), or an unpaired two tailed t-test g, *p ≤ 0.05, **p ≤ 0.01).
