Extended Data Fig. 6: ZNF512 and ZNF512B utilize their zinc fingers to target the pericentric sequences.

(a) Schematic diagram of ZNF512 and ZNF512B protein sequences. Zinc finger domains and linkers’ lengths were labeled. (b) Multi-sequence alignments of zinc finger domains in mouse ZNF512 and ZNF512B. The zinc fingerprint residues at positions −1, 3, 6 of each C2H2 zinc finger were highlighted. (c) The binding motifs of mouse ZNF512 and ZNF512B were predicted by a polynomial SVM-based algorithm. (d) Representative cross-section fluorescence images showing the relative localization of EGFP-ZNF512 with one or two zinc fingerprints mutated and DAPI in Znf512, Znf512b, Suv39h1, Suv39h2 quadruple KO (QKO) cells. Zinc fingerprint residues mutated versions, in which one zinc finger of ZNF512 mutated to AAA, were abbreviated as ZF-3A. Zinc fingerprint residues mutated versions, which two zinc fingers of ZNF512 mutated to AAA, were abbreviated as ZF-6A. Scale bar, 5 μm. (e) Representative cross-section fluorescence images showing the relative localization of EGFP-ZNF512B with one or two zinc fingerprints mutated and DAPI in Znf512, Znf512b, Suv39h1, Suv39h2 quadruple KO (QKO) cells. Zinc fingerprint residues mutated versions, which one zinc finger of ZNF512B mutated to AAA, were abbreviated as ZF-3A. Zinc fingerprint residues mutated versions, which two zinc fingers of ZNF512B mutated to AAA, were abbreviated as ZF-6A. Scale bar, 5 μm. Data are representative of three (d) or one (e) independent biological experiments.