Extended Data Fig. 10: Single-cell ATAC analysis of patient CAR T cell response to IL-4.
From: Single-cell CAR T atlas reveals type 2 function in 8-year leukaemia remission
a, Volcano plot showing differential accessible peak activities in BCA2 or 1 CAR T cells with and without the addition of 10 ng/mL IL-4. b, Pseudo-bulk chromatin accessibility tracks in the genomic region of top-ranked genes upregulated in 10 ng/mL IL-4 treated BCA2 or 1 CAR T cells compared to the original condition, including type 1 marker gene, type 2 marker gene, cytotoxicity marker genes, chemokine marker genes or activation/proliferation marker genes, depicted separately for each patient with and without the addition of IL-4. c, Pseudo-bulk chromatin accessibility tracks in the genomic region of CSF2 and Il32, depicted separately for each patient with and without the addition of IL-4. d, Expression profile of type 2 motif MA0037.3 (GATA3) and type 1 motif MA0690.1 (STAT1) across each patient with and without the addition of IL-4. In b and c, the enhancer elements predicted by ENCODE within the region of each gene are highlighted in a light-grey shade. Significance levels were calculated with two-tailed Mann-Whitney test (a).
