Extended Data Fig. 5: Capsaicin does not alter growth or invasion of cancer cells in vitro and reduces tumour growth in immune-compromised mice in vivo.
From: Neuronal substance P drives metastasis through an extracellular RNA–TLR7 axis
(a) Sensory (CGRP +) and sympathetic (TH +) innervation in ipsilateral or contralateral mammary glands 7 days after capsaicin administration. (b) Quantification of CGRP+ nerve bundles post-capsaicin administration. *p = 0.0403, t-test. Mean ± SD. (c-d) 3D culture of 4T1 spheroids in the presence of capsaicin (1–100 μM). (c) Invasion quantification. nsp > 0.9999, Kruskal-Wallis test. (d) Spheroid surface area. nsp = 0.7108, Kruskal-Wallis test. Mean ± SD. (e-f) Sensory-specific denervation of 4T1 tumours grown in NSG mice. (e) Schematic. (f) Tumour growth. ****p < 0.0001, t-test. Mean ± SEM.
