Extended Data Fig. 7: IIO cultures provide a route to study ICI-induced colitis.
From: Human organoids with an autologous tissue-resident immune compartment
a, Bihourly quantification of caspase 3/7 signal in ICI- or isotype- treated IIOs or matched PBMC-organoid co-cultures. 3-independent co-cultures from two biological replicates are shown. Mean and SD. b, LDH signal in the supernatant of the conditions described in a after 48 h of culture. Two-tailed paired T test. c, Flow cytometry quantification of the number of cytotoxic CD38+ Ki67+ GzmB+ CD8+ T cells within the conditions described in a after 48 h of culture. d, Boolean gating-derived flow cytometry plots showing the counts of CD38+ GzmB+ Ki67+ “pathogenic” CD8+ T cells and allogeneic DCs after 48 h of IIO culture in each of the TRM donors, treated with either an isotype control or ICI e, Flow cytometry quantification of the number GzmB+ T cells within the conditions described in a after 48 h of IIO culture. ICI immune checkpoint inhibitor, R1 replicate 1, R2 replicate 2. DC dendritic cell.
