Fig. 1: MICL is required for control of neutrophil responses.
From: Recognition and control of neutrophil extracellular trap formation by MICL
a, tSNE plots of CD45+ myeloid populations in the inflamed ankle joint during CAIA displayed as CD11b+ cells (grey), neutrophils (blue), Ly6Chigh cells (green), Ly6Clow cells (orange) and remaining antigen-presenting cells (APCs; pale violet). b, Myeloid cell populations (defined as shown in the gating strategy in Extended Data Fig. 1b) in the inflamed ankle joint during CAIA at day 7 are represented as a percentage of total live cells (pooled data from two independent experiments with four mice per group per experiment). WT versus Micl−/− neutrophils P < 0.0001. c, Schematic representation of the anti-Ly6G-mediated neutrophil depletion strategy in the CAIA model. D0, day 0; LPS, lipopolysaccharide. d, Quantification of neutrophils (CD45+CD11b+F4/80−SSChigh) in the peripheral blood at day 9 by flow cytometry (n = 1 experiment with 3 mice per group). Isotype versus anti-Ly6G WT P = 0.0035 and Micl−/− P = 0.0021. e, Severity scoring of WT and knockout (Micl−/−) mice treated with isotype or anti-Ly6G antibodies (Abs), as indicated (n = 1 experiment with 5 mice per group). f, Schematic representation of neutrophil adoptive transfer during CAIA in WT mice. KO, knockout. g, CAIA severity scoring in WT mice that received adoptively transferred WT or knockout neutrophils, as indicated (pooled data from two independent experiments with five mice per group per experiment). Day 8 WT versus Micl−/− P = 0.0013. h, Representative Safranin O-stained sections of the tarsal joints of WT mice that received adoptively transferred WT or knockout neutrophils, as indicated at day 8 (left). Synovial inflammation (black asterisks) is indicated. Scale bars, 500 µm. The histological arthritis severity score is also shown (right; nine mice per group). WT versus Micl−/− P = 0.0281. Data are represented as mean ± s.d. (b,d,e,g,h). Statistical significance was determined by two-way analysis of variance (ANOVA) with Bonferroni’s multiple comparisons test (b,d,e,g). Data were analysed using an unpaired two-tailed Student’s t-test (h). *P < 0.05, **P < 0.01 and ****P < 0.0001. Schematics in panels c,f were created using BioRender (https://biorender.com).
