Extended Data Fig. 1: MICL regulates inflammation during arthritis.
From: Recognition and control of neutrophil extracellular trap formation by MICL
a, Schematic of the CAIA model and severity scoring shown as mean ± SEM (pooled data from three independent experiments, n = 16 biologically independent mice) assessed over time. Statistical significance was determined by two-way ANOVA with Bonferroni’s multiple comparisons test. Schematic in panel a was created using BioRender (https://biorender.com). b, Flow cytometry gating strategy used for characterisation of the ankle joint cellular infiltrate. Cells isolated from the inflamed joint were first gated to identify single viable cells using a fixable viability dye. CD11b+ populations were further gated into subsets namely; neutrophils (Ly6G+CD11b+) and Ly6Chigh cells (Ly6ChighF4/80+CD11b+) or Ly6Clow cells (Ly6ClowF4/80+CD11b+). Antigen presenting cells (APCs) were gated as MHC II+CD11b+. c, Neutrophils and Ly6Chigh (defined as shown in the gating strategy in Extended Data Fig. 1b) in the ankle joint during CAIA at day 5 are represented as a percentage of total live cells (n = 1 experiment with 6 mice/group). Statistical significance determined by two-way ANOVA with Bonferroni’s multiple comparisons test. WT, wild-type mice; *p < 0.05.
