Extended Data Fig. 7: smFISH quantification analysis.
From: Cellular communities reveal trajectories of brain ageing and Alzheimer’s disease
(a) Gene expression (columns) across microglial subpopulations (rows) for selected differential genes and known markers. Dot colour: column-scaled mean expression of expressing cells. Dot size: percentage of expressing cells. (b) Gene expression of markers used for smFISH across microglial subpopulations. Dot colour and size as in (a). (c) A gallery of representative RNAscope smFISH data showing split channels by marker: CPMhigh Mic.12, TPRG1high Mic.13, and a MRC1high macrophage, together with IBA1 (green) and DAPI (blue) staining. (d) Bivariate expression distributions in snRNA-seq (top) and smFISH (bottom), coloured by assigned snRNA-seq subpopulations (top) predicted assignment in smFISH data (bottom, Methods).
