Extended Data Fig. 4: Neuronal activation in different brain regions after puerarin administration.
From: A brain-to-gut signal controls intestinal fat absorption
a, Chemical structure of puerarin molecule. b-j, cFOS immunostaining of brain regions in mice treated with i.p. injection of puerarin and vehicle under pair-feeding HFD, and counts of c-fos positive neurons in various rostral-caudal nucleus (n = 4 per group), including striatum (CPu) and bed nucleus of stria terminalis (BST) (b), paraventricular hypothalamus (PVH) (c), dorsomedial hypothalamus (DMH) (d), ventromedial hypothalamus (VMH) and arcuate nucleus of hypothalamus (ARC) (e), lateral hypothalamus (LH) (f), basolateral and central amygdaloid nucleus (BLA and CeA, Amygdala) (g), ventral tegmental area (VTA) and substantia nigra (SN) (h), dorsomedial, dorsolateral, lateral and ventrolateral periaqueductal gray (PAG including DMPAG, DLPAG, LPAG, and VLPAG) (i), and parabrachial nucleus (PBN) (j). Scale bar, 500 μm (b, h), 200 μm (d-g, i-j) and 100 μm (c). Data were analyzed using two-tailed Student’s t test and are presented as mean ± S.E.M. Veh, vehicle; Pue, puerarin. ns, no significance. Detailed statistics see source data.
