Extended Data Fig. 5: Mutation burdens and driver mutations.

a, Dot plot showing the corrected single nucleotide variant mutation burden of HSPCs from donors against donor age. Solid black line shows the results of a linear regression of this relationship, with the grey shaded area the 95% confidence interval. b, Dot plot showing the number of additional mutations in recipient colonies, after bioinformatically removing burdens associated with the APOBEC (N4) and platinum chemotherapy (N3) signatures that are sporadic. Where there are multiple HSPCs from a single expansion, only one colony per individual is used for this inference, as the burdens are not independent. Circles denote the point estimate and error bars indicate the 95% confidence intervals calculated from n = 2,824 independent colonies. c, Stacked bar plot showing the total numbers of independent driver mutations detected per gene, coloured by mutation consequence. d, Heatmap showing the number of independent driver mutations per gene in each pair. The far right column shows the total number of drivers in each individual across genes. e, Bar plot showing the possible molecular times of acquisition of each driver mutation. Bars are grouped and coloured by gene. SNV, single nucleotide variant; HCT, haematopoietic cell transplantation.