Extended Data Fig. 3: Mutational signatures.

a, 96-profile mutational signatures of mutational processes active in HSCs/HSPCs, as extracted using a hierarchical dirichlet process (Methods). Interpretation of each signature, by comparison with COSMIC signatures, is shown to the right of each profile. b, Stacked barplot showing the absolute contribution of each signature to each sample. Each column is a single sample, with samples grouped by pair. Tiles below the columns indicate whether the sample is from the donor (green) or recipient (orange). Three outlier samples in the Pair 10 recipient had extremely high burdens and these have been attenuated to aid visualization. c, Box-and-whisker plot showing the per sample burden of N3 mutations (platinum-associated signature), divided by pair and donor/recipient origin. Lines show the median values, boxes show the interquartile range, and whiskers the range for the n = 10 independent donor-recipient pairs. d, Bar plot showing the percentage of HSPCs from each donor (green) and recipient (orange) that are “positive” for the SBS2 signature (APOBEC-associated, ≥10 mutations). e, Jittered dot plot showing the absolute burden of SBS2 mutations in positive samples. Points are coloured by pair, and are either triangles (recipient origin) or circles (donor origin). HSPC, haematopoietic stem or progenitor cell; SBS, single base substitution.