Extended Data Fig. 11: Mechanism of LXR mediated anti-tumour effects.
From: Liver X receptor unlinks intestinal regeneration and tumorigenesis
(a, b) WT mice were fed with either standard or GW3965 diet and exposed to AOM-DSS tumour model. Starting from d22 after AOM injection, mice were treated with repeated (weekly) intraperitoneal injections of either 300 µg anti-CD19 (αCD19) (a) or anti-CD8 (αCD8) (b) antibody. Control mice were injected with PBS. Mice were sacrificed at day 70 and macroscopic tumour numbers were counted. Quantification of tumour numbers (log2 normalized) and sizes are shown. (c) Expression levels of CYP27A1, ABCG1, ABCA1, CD19, MS4A1, CR2, CD8A, GZMA, and CD69 in a human CRC whole genome transcriptome array dataset stratified in six tumour subtypes (c1 to c6) and non-tumour control (NT). Data are representative of two independent experiments (a,b) with 4–8 mice per condition (each dot represents one mouse). Data are shown as mean ± s.e.m. (a,b) and quartiles (c). Significance was assessed by One-way ANOVA with Tukey’s post-hoc test in (a,b) or two-way ANOVA with Dunnett’s post hoc test in (c). Part of the schematics in panels a and b were adapted from ref. 19, CC-BY 4.0.
