Extended Data Fig. 8: Dietary restriction of valine alters valine concentration and HDAC6 nuclear ___location.
From: Human HDAC6 senses valine abundancy to regulate DNA damage
a–c, Absolute quantification of amino acids in the plasma of the mice fed on diets containing different concentrations of valine was done using targeted metabonomics. d–f, Absolute quantification of amino acids in the tumour tissue of the mice fed on diets containing different concentrations of valine was done using targeted metabonomics. g, h, Tumour images (g) and volume (h) of HCT116 cell-derived tumours in nude mice fed on a special diet containing four concentrations of valine. i, Tumour weight at the end point from g. j, Body weight of nude mice in g. k, Food intake in g. l, m, Body weight of nude mice grafted with CRC PDX on valine restriction diets in the prevention group (l) and treatment group (m). n, o, Representative immunostaining images (n) and quantification (o) of subcellular localization of HDAC6 in the HCT116 cell-derived tumour sections. Scale bar, 10 μm. p, Quantification for γH2AX immunofluorescence staining for γH2AX (red) of tumour sections in g. For a–f, h–m, o and p, data are presented as mean ± s.d.. For a, d, e, f, l, m, n = 6 mice. For b, c, h, i, j, n = 8 mice. For o, n = 3 independent experiments. For p, n = 100 microscopic views examined for 8 mice. Statistical analysis was performed using one-way ANOVA (a, d, h–m, o), two-way ANOVA (b, c, e, f) and Mann–Whitney U-test (p); *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, NS, not significant. For gel source data, see Supplementary Fig. 1.
