Extended Data Fig. 5: CD34+ cell output towards mature lineages.
From: Long-term lineage commitment in haematopoietic stem cell gene therapy
(A) Dynamics of the lineage output over time (x-axis) calculated with the sharing ratio (y-axis) between CD34+ cells and each lineage (different colors) expressed as percentage normalized with Z-score by patient and time point, reported by clinical study. Spline curves with CI 0.75. (B) Similar to (A), dynamics of the lineage output (y-axis) over time (x-axis) stratified by patient age (0–2 years, 2–15 years, >30 years). (C) Lineage output for XSCID patients (N = 10) under LV-HSPC gene therapy treatment (De Ravin S., et al. Nature Communications 2022). (D) Box-plots of sharing ratio (bar indicates median, whisker 25–75 percentiles), normalized by Z-score (y-axis) isolated in all patients from 24 months and averaged, in BM and PB tissues (circle or triangular dot shapes), grouping cell markers (colors) by cell lineages, and stratified by patient’s age within each clinical trial. Statistical tests are performed comparing the different lineages (Kruskal Wallis test). The bars represent the median, the whiskers extend to 1.5 times the IQR, and the p-value threshold is set at 0.05. (E) Similar to (D), box-plot representation of normalized sharing ratio (y-axis) stratified by lineage within each clinical trial, comparing the different groups of treatment age (Kruskal Wallis test).
