Fig. 1: Tumour-infiltrating T cells are restimulated in permissive TMEs.
From: Cancer cells impair monocyte-mediated T cell stimulation to evade immunity
a, Schematic of subcutaneous injection of YUMM1.7OVA NTT cells or RTT cells in Rag2–/– mice and OT-1Luc ACT through intravenous (i.v.) injection. b, Left, ACT responses of NTT and RTT tumours (n = 5 mice per group). Right, representative bioluminescence imaging (BLI) pictures of T cells 96 h after ACT; key indicates radiance (photons per second that leave a square centimeter of tissue and radiate into a solid angle of one steradian). c, Uniform manifold approximation and projection (UMAP) maps of scRNA-seq of CD45+ cells in NTT and RTT tumours 72 h after ACT (n = 4 tumours pooled per group). d, Relative cell frequencies from scRNA-seq. e, Representative multiparameter IF images of YUMM1.7OVA NTT and RTT tumours 48 h after ACT (n = 3 tumours per group). Scale bar, 400 µm (zoom-ins, 50 µm and 10 µm). f, Relative T cell frequency and distance to the next immune cell in NTT tumours (n = 3 tumours per group). g, Representative IF image of Nur77–GFP OT-1 cells in YUMM1.7OVA NTT and RTT tumours 48 h after ACT (n = 3 tumours per group). Scale bar, 10 µm. Arrows indicate Nur77+ OT-1 cells. h, Representative IF image of YUMM1.7OVA NTT and RTT tumours 72 h after ACT. Dashed lines depict the tumour border (n = 2 tumours per group). Scale bar, 500 µm. i, Left, quantification of T cells by BLI over time after intratumoral (i.t.) ACT (mean ± s.e.m., n = 4 mice per group). Two-way analysis of variance (ANOVA) with Sidak’s multiple comparisons test. Right, representative BLI image 96 h after ACT (key as in b). j, T cell states by flow cytometry 120 h after i.t. ACT (mean ± s.e.m., n = 5 NTT, n = 4 RTT tumours). k, Effector memory T cell signature29 on scRNA-seq of T cells (n = 14 NTT, n = 18 RTT pooled tumours). l, Left, representative BLI images of T cells in NTT and contralateral (CL) RTT tumours after i.t. ACT (key as in b). Right, growth curves (mean ± s.e.m., n = 6 mice per group). Arrows in b and l indicate day of ACT.
