Extended Data Fig. 1: Characterization of immune permissive versus suppressive TMEs in mouse melanoma models.
From: Cancer cells impair monocyte-mediated T cell stimulation to evade immunity
a, Representative images of bioluminescent live imaging (BLI) of T cell infiltration after adoptive CD8+ OT-1Luc T cell transfer (ACT) in YUMM1.7OVA NTT and RTT tumors. b, Scheme outlining the in vitro killing of NTT and RTT cells with activated CD8+ OT-1 T cells (1:1 ratio) (left), histograms (flow cytometry) displaying MHCI levels of NTT and RTT cells (middle) and normalized ratio of 50:50 NTT/RTT cells mixed cultures in an in vitro killing assay with CD8+ OT-1 T cells. Normalization was performed with NTT and RTT percentages from the same 50:50 mixed cultures without CD8+ OT-1 T cells to account for proliferation differences (n = 3 biological replicates) (right). Two-tailed unpaired Student’s t-test. c, UMAP of scRNA-seq of CD45+ cells of YUMM1.7OVA NTT and RTT tumors in Rag2−/− before ACT (n = 3 tumors pooled/group). d, Relative frequency of cell types across conditions in YUMM1.7OVA tumors post-ACT (n = 4 tumors pooled/group). e, Expression of myeloid cell markers in each cluster (scRNA-seq). f, Heatmap of scaled gene expression (scRNA-seq) across cell clusters between NTT and RTT YUMM1.7OVA tumors post-ACT. g, Response to immune checkpoint blockade (ICB, anti-PD1/CTLA-4) of YUMM3.3 NTT and RTT tumors (n = 6 NTT, n = 7 NTT + ICB, n = 8 RTT, n = 9 RTT + ICB). Two-way ANOVA with Tukey’s multiple comparison. h, UMAP of scRNA-seq of CD45+ cells of YUMM3.3 NTT and RTT (n = 1 tumor/group). i, Relative frequency of cell types across conditions. j, Heatmap of scaled gene expression (scRNA-seq) across cell clusters between YUMM3.3 NTT and RTT. Bar graphs and growth curves depict the mean ± s.e.m. ns = not significant.
