Extended Data Fig. 9: TRiC clusters analysis in untreated and treated datasets.
From: In situ analysis reveals the TRiC duty cycle and PDCD5 as an open-state cofactor
a, Illustration of ribosome exit tunnel side (ETS) and non-ETS for the analysis in (b, j). The position of exit tunnel was highlighted by a yellow dot. b, Distribution of the nearest neighbouring TRiC of ribosomes within 70 nm (TRiC centre to ribosome centre) in untreated cells. c,k, Percentages of open and closed TRiC in the ETS of ribosomes. The data represents mean ± SD of three independent data collection sessions. 2,716 open and 2,900 closed TRiC in untreated cells (c). 3,453 open TRiC and 2,924 closed TRiC in treated cells (k). Statistical analysis was conducted using two-tailed unpaired t-tests. ns, not significant (p > 0.05); ***P = 0.0002; ****P < 0.0001. d,e, The abundance of the closed TRiC within clusters and non-clusters in untreated (d) and HHT-treated cells (e). The percentage of TRiC in cluster was calculated as closed-TRiC in clusters (1,215 particles for untreated, 647 for treated) divided by all closed TRiC (4,054 for untreated, 3,418 for treated). The cluster length means the particle number per TRiC cluster. f,g, The number of open TRiC with neighbouring closed TRiC at the indicated distance in untreated (f) and treated (g) cells. Open TRiC particles with neighbouring closed TRiC at various distances (15 to 40 nm) were counted. h,i, Centre-to-centre distance of TRiC pairs in clusters of varying lengths. The numbers (N) are N2 (cluster length = 2) = 326 particles, N3 = 218 particles, N4 = 74 particles, N5 = 35 particles, N6 = 16 particles and N7 = 4 particles in untreated cells, and N2 = 195 particles, N3 = 116 particles, N4 = 27 particles, N5 = 7 particles and N6 = 4 particles in treated cells. The data represent the mean ± SD (Methods). j, The analysis of ribosomes and TRiC in HHT-treated cells, similar to (b).
