Fig. 4: Translation inhibition diminishes substrate in all states and clusters of closed TRiC reduce in HHT-treated cells.
From: In situ analysis reveals the TRiC duty cycle and PDCD5 as an open-state cofactor
a, TRiC states mapped into a tomogram of translation-inhibited (HHT treated) cells. Clusters are not apparent. b, Quantification of TRiC complexes belonging to clusters at thresholds ranging from 15 to 40 nm (centre-to-centre distance) in untreated cells. The analyses were performed for 4,054 closed TRiC (left) and 3,353 open TRiC (right) particles from 360 tomograms. c, The counts of detected TRiC clusters within 15 to 40 nm (centre-to-centre distance) in treated cells. The analyses were performed for 3,418 closed TRiC (left) and 3,785 open TRiC (right) particles from 352 tomograms. d, Classes of TRiC states obtained by subtomogram averaging in HHT-treated (translation inhibited) cells. Densities in the substrate position are shown in yellow. The substrate densities were Gaussian filtered (sDev = 4) for visualization. e,f, Quantification of the TRiC state distribution under untreated (e) and HHT-treated (f) conditions. Asterisks in d–f indicate reduced densities in the chamber of TRiC.
