Fig. 5: The in vivo TRiC folding cycle in human cells.
From: In situ analysis reveals the TRiC duty cycle and PDCD5 as an open-state cofactor
Schematic of the proposed TRiC duty cycle inside human cells. Substrates are delivered to TRiC by PFD or directly enter the chamber of open TRiC. Open TRiC is abundant and not observed without PDCD5 and substrate (for the latter unless translation is inhibited). ATP hydrolysis drives TRiC closure, PDCD5 dissociates and closed TRiC tends to form linear clusters. Once substrates are folded, TRiC opens, and the substrates are released. PDCD5 binds to the open TRiC complex to initiate a new cycle. The abundance of TRiC states as observed in our analysis is listed for each step of the cycle.
