Extended Data Fig. 1: Open and closed TRiC structures in human cells.
From: In situ analysis reveals the TRiC duty cycle and PDCD5 as an open-state cofactor
a, A tomogram slice from an untreated cell. Scale bar, 100 nm. The micrograph is representative of 360 similar tomograms. b, TRiC densities in the tomogram slices and corresponding template matching peaks colour-coded by constrained cross-correlation (CCC). Scale bar, 30 nm. Potential TRiC particles were extracted based on the CCC value from template matching. The micrograph is representative of 360 similar tomograms. c, Open TRiC map (C1 symmetry) fitted with PDB 7X3J. Arrows point to the additional density: ring 1 (green); ring2 (cyan). d,e, Closed TRiC maps with D8 symmetry (d) and C1 symmetry (e) were fitted with atomic models from PDB. f, The open TRiC map fitted with PDB 7X3J. The resolution does not allow us to assign ATP or ADP to the map but secondary structure is resolved. g, Fourier shell correlation (FSC) curves of TRiC maps (c-e). h, Percentages of open and closed TRiC in cells growing in the normal condition. i, Evaluation of processed and unprocessed potential TRiC particles in this study. Particle coordinates from the subtomogram averaging were mapped back into the tomogram, and the TRiC-like (based on size and shape) particles that were not processed were manually counted in twenty tomograms. In total, 17 TRiC-like particles were not processed, while 515 particles were processed.
