Extended Data Fig. 3: TRiC states fitted with atomic models in untreated cells.
From: In situ analysis reveals the TRiC duty cycle and PDCD5 as an open-state cofactor
a, The cryo-ET map of open TRiC without PFD bound in untreated cells. The densities within the TRiC cavity were Gaussian-filtered (sDev = 4). b, Different views of the map in (a) showing both PDCD5 and potential substrate densities. The predicted model of PDCD5-CCT3-CCT1-CCT4 was fitted into the map in (a). c, The open TRiC structure from EMDB (EMD-13754). The map was Gaussian-filtered (sDev = 4) for visualization. d, Different views of EMD-13754 showing potential substrate densities but no PDCD5 density. The AlphaFold-Multimer predicted model of PDCD5-CCT3-CCT1-CCT4 was fitted into the open TRiC map in (c). e, Atomic model from PDB 7WU7 was fitted into the open TRiC structure associated with 1 PFD. The chamber densities were Gaussian-filtered (sDev = 4). f, PFD (PDB 7WU7) was fitted into the corresponding densities segmented from (e). g, Different views of the map in (e). The predicted model of PDCD5-CCT3-1-4 was fitted into the structure in (e). h, The open TRiC bound with 2 PFDs was fitted with PDB 7WU7. i, PFD densities segmented from (h) were fitted with PDB 7WU7. j, Different views of the map in (h). The predicted model of PDCD5-CCT3-CCT1-CCT4 was fitted into the map in (h).
